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Today: 28 February 2020, Friday.

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#1

 

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Description Lipoyl synthases are key enzymes in lipoic acid biosynthesis, a co-factor of several enzyme complexes involved in central metabolism. Plant pyruvate dehydrogenase complex (PDH), located in mitochondria and plastids, catalyses the first step of fatty acid biosynthesis in these organelles. Among their different components, the E2 subunit requires the lipoic acid prosthetic group to be active. De novo lipoic acid biosynthesis is achieved by the successive action of two enzymes on octanoyl-ACP: octanoyltransferase (LIP2) and lipoyl synthase (LIP1). In this study, two plastidial lipoyl synthase genes from sunflower (Helianthus annuus L.) were identified (HaLIP1p1 and HaLIP1p2), sequenced and cloned in a heterologous production system (Escherichia coli). Gene expression studies revealed similar expression patterns for both isoforms, with a slight predominance of HaLIP1p1 in vegetative tissues and mature seeds. Tertiary structural models for these enzymes indicate they both have the same theoretical catalytic sites, using lipoyl-lys and 5-deoxyadenosine as docking substrates. The fatty acid profile of E. coli cells overexpressing HaLIP1p1 and HaLIP1p2 did not present major differences, and the in vivo activity of both proteins was confirmed by complementation of an E. coli JW0623 mutant in which lipoyl synthase is defective. Although no significant differences were detected in the total fatty acid composition of transgenic Arabidopsis thaliana seeds overexpressing any of both proteins, a lipidomic analysis revealed a redistribution of the glycerolipid species, accompanied with increased phosphatidylethanolamine (PE) content and a decrease in diacyglycerols (DAG) and phosphatidylcholine (PC). Depletion of the SAM co-factor caused by HaLIP1p1 and HaLIP1p2 overexpression in transgenic plants could explain this remodelling through its effects on PC synthesis.

#Sport & Camping
Field # Sport & Camping
Updated 28 February 2020

#2

 

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Description You can take (almost) every fitness class you could ever want right from your living room.

#Sport & Camping
Field # Sport & Camping
Updated 27 February 2020

#3

 

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Description Psychologist Dr Elisa Raffaella Ferrè wants to know how a lack of gravity will change the way we think.

#Sport & Camping
Field # Sport & Camping
Updated 27 February 2020

#4

 

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Description (Inside Science) -- In the ancient Greek legend, the messenger Pheidippides ran from the battlefield of Marathon to Athens -- a distance of more than 20 miles -- to bring news of the Greek's victory over the Persians in the battle of Marathon. Then he collapsed and died. The race now known as a marathon was developed for the first modern Olympic Games in Greece in 1896 and its

#Sport & Camping
Field # Sport & Camping
Updated 26 February 2020

#5

 

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Description Through a combination of single crystal growth, experiments involving in situ deposition of surface adatoms, and complimentary modeling, we examine the electronic transport properties of lithium-decorated ZrTe5 thin films. We observe that the surface states in ZrTe5 are robust against Li adsorption. Both the surface electron density and the associated Berry phase are remarkably robust to adsorption of Li atoms. Fitting to the Hall conductivity data reveals that there exist two types of bulk carriers: those for which the carrier density is insensitive to Li adsorption, and those whose density decreases during initial Li depositions and then saturates with further Li adsorption. We propose this dependence is due to the gating effect of a Li-adsorption-generated dipole layer at the ZrTe5 surface.

#Sport & Camping
Field # Sport & Camping
Updated 26 February 2020

#6

 

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Description TBD

#Sport & Camping
Field # Sport & Camping
Updated 25 February 2020

#7

 

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Description Totally worth giving up our rights for.

#Sport & Camping
Field # Sport & Camping
Updated 24 February 2020

#8

 

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Description Over the past decade almost US$200 billion has been invested globally in mobility technology that promises to improve our ability to get around. More than US$33 billion was invested last year alone. Another measure of interest ...

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Field # Sport & Camping
Updated 24 February 2020

#9

 

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Description And there's not much they can do about it.

#Sport & Camping
Field # Sport & Camping
Updated 22 February 2020